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Purification and Properties of Alanine aminotransferase from Germinating Castor Bean Endosperms
https://doi.org/10.24729/00009212
https://doi.org/10.24729/000092124fdfc5ee-986e-4302-8ed2-ef6b204d625a
名前 / ファイル | ライセンス | アクション |
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KJ00004350446.pdf (579.7 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2009-08-25 | |||||
タイトル | ||||||
タイトル | Purification and Properties of Alanine aminotransferase from Germinating Castor Bean Endosperms | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.24729/00009212 | |||||
ID登録タイプ | JaLC | |||||
著者 |
FUJII, Michiko
× FUJII, Michiko× YOSHII, Keiji |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Alanine aminotransferase from germinating castor bean endosperms was purified about 1,500-fold by ammonium sulfate fractionation and chromatographies with DEAE-Sepharose CL-6B, TEAE-Cellulose, QAE-Sephadex A-50, and TSK-5PW (HPLC). The molecular weight of the enzyme was about 110kDa, with two identical subunits. The purified enzyme was highly specific for alanine as an amino donor and 2-oxoglutarate as an acceptor, and it showed also glyoxylate transamination activities at low rate. The evidence indicates that alanine: 2-oxoglutarate aminotransferase and glyoxylate aminotransferase activities are associated with the same protein. Pyridoxal phosphate as a cofactor was so tightly bound to the enzyme that added pyridoxal phosphate was not required in the assays. The enzyme showed normal Michaelis-Menten kinetics for all substrates. Reactions proceeded by a Ping Pong Bi Bi mechanism. The K_m for L-alanine, 2-oxoglutarate were 3.4mM and 0.26mM, respectively. The optimum pH was 8.5 and pI was about 4.6. | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Bulletin of the University of Osaka Prefecture. Ser. B, Agriculture and life sciences. 1995, 47, p.95-104 | |||||
書誌情報 |
Bulletin of the University of Osaka Prefecture. Ser. B, Agriculture and life sciences 巻 47, p. 95-104, 発行日 1995-03-31 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0366-3353 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10923100 | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | University of Osaka Prefecture |