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Purification and Properties of Glutamine Synthetase from Germinating Castor Bean Endosperm
https://doi.org/10.24729/00009260
https://doi.org/10.24729/00009260df43e993-09b9-40ba-acb1-bafc2c4872ae
名前 / ファイル | ライセンス | アクション |
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KJ00004361227.pdf (547.4 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2009-08-25 | |||||
タイトル | ||||||
タイトル | Purification and Properties of Glutamine Synthetase from Germinating Castor Bean Endosperm | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.24729/00009260 | |||||
ID登録タイプ | JaLC | |||||
著者 |
FUJII, Michiko
× FUJII, Michiko× NAKAJIMA, Kenji |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The activity of glutamine synthetase in germinating castor bean endosperm resides in the cytosol. The activity was found to increase rapidly the 3rd to 4th day of germanating in the dark and to be a peak at 5th day. The enzyme was purified from 5-day-old endosperm approximately 100-fold to apparent homogeneity. Electrophoresis in polyacrylamide gels containing sodium dodecyl sulfate yeilded a single band with a molecular weight of 55,000, suggesting that subunits of GS have a identical molecular weight. The Km is 4.9mM for L-glutamate, 0.65mM for NH_2OH, 0.92mM for NH_4Cl (biosynthetic assay), 34.1mM for L-glutamine and 6.28mM for NH_2OH (transferase assay). ATP acts as a sigmoidal substrate with a "S_<0.5>" of 1.4mM. Inhibition of the enzyme is observed with AMP, ADP, CTP, GTP, ITP, L-glycine, L-histidine, citrate, carbamyl phsphate, and glucoseamine-6-phosphate. The energe charge significantly affected the activity of GS. | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Bulletin of the University of Osaka Prefecture. Ser. B, Agriculture and biology. 1992, 44, p.89-97 | |||||
書誌情報 |
Bulletin of the University of Osaka Prefecture. Ser. B, Agriculture and biology 巻 44, p. 89-97, 発行日 1992-03-31 |
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書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00106812 | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | University of Osaka Prefecture |