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Methanethiol was formed only in the headspace of the residue but not in the filtrate or supernatant fractions like dimethyl disulfide. Freeze-thawing, however, suppressed their formation in all homogenate fractions. The immersion of the fresh residue in a liquid medium decreased the concentration of methanethiol which indicates that this compound is highly likely soluble in water and produces only in the headspace of the environment wherein the condition is free of liquid medium. Hence, methanethiol formation was not possible except for dimethyl disulfide after addition of the co-factor, pyridoxal 5-phosphate, in the frozen residue. Both compounds were also inhibited by aminooxyacetic acid, a potential inhibitor of pyridoxal phosphate-dependent enzymes, which indicates that MT and DMDS are produced upon the action of cysteine sulfoxide lyase. 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<Original>Suppression of Volatile Sulfur Compounds from Broccoli by Freeze-Thawing and Their Mechanism with Filtration and Centrifugal Fractionation
https://doi.org/10.24729/00009667
https://doi.org/10.24729/0000966783b188ae-4dca-49f0-b05e-d6124984aff1
名前 / ファイル | ライセンス | アクション |
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KJ00000052076.pdf (885.1 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2009-08-25 | |||||
タイトル | ||||||
タイトル | <Original>Suppression of Volatile Sulfur Compounds from Broccoli by Freeze-Thawing and Their Mechanism with Filtration and Centrifugal Fractionation | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | broccoli | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cysteine sulfoxide lyase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | dimethyl disulfide | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | freeze-thawing | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | residue fraction | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.24729/00009667 | |||||
ID登録タイプ | JaLC | |||||
著者 |
TULIO, Jr.
× TULIO, Jr.× UEDA, Yoshinori× YAMANAKA, Hiroyuki× CHACRIN, Kazuo |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The formation of methanethiol and dimethyl disulfide in the broccoli tissue homogenates following filtration and centrifugal fractionation, and their suppression by freeze-thawing was investigated. Methanethiol was formed only in the headspace of the residue but not in the filtrate or supernatant fractions like dimethyl disulfide. Freeze-thawing, however, suppressed their formation in all homogenate fractions. The immersion of the fresh residue in a liquid medium decreased the concentration of methanethiol which indicates that this compound is highly likely soluble in water and produces only in the headspace of the environment wherein the condition is free of liquid medium. Hence, methanethiol formation was not possible except for dimethyl disulfide after addition of the co-factor, pyridoxal 5-phosphate, in the frozen residue. Both compounds were also inhibited by aminooxyacetic acid, a potential inhibitor of pyridoxal phosphate-dependent enzymes, which indicates that MT and DMDS are produced upon the action of cysteine sulfoxide lyase. This enzyme, which yielded strong activity in the residue upon extraction with buffer containing Triton X-100, is highly likely to be a bound enzyme. Suppression of methanethiol and dimethyl disulfide during freeze-thawing is likely attributed to the retardation of the enzyme action due in part to the loss of the co-factor, pyridoxal 5- phosphate and its substrate, S-methyl-L-cysteine sulfoxide and owing to the solubility of methanethiol and its affinity for gaseous condition. | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 大阪府立大学大学院農学生命科学研究科学術報告. 2003, 55, p.15-22 | |||||
書誌情報 |
大阪府立大学大学院農学生命科学研究科学術報告 巻 55, p. 15-22, 発行日 2003-03-31 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1346-1575 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11576192 | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University |