{"created":"2023-05-15T13:53:32.001888+00:00","id":9219,"links":{},"metadata":{"_buckets":{"deposit":"fdcb197e-d27b-4189-8f17-6b06797a815f"},"_deposit":{"created_by":1,"id":"9219","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"9219"},"status":"published"},"_oai":{"id":"oai:omu.repo.nii.ac.jp:00009219","sets":["1549:1661:1662:1668:1672","2062:130"]},"author_link":["22037","22038"],"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1995-03-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"104","bibliographicPageStart":"95","bibliographicVolumeNumber":"47","bibliographic_titles":[{"bibliographic_title":"Bulletin of the University of Osaka Prefecture. Ser. B, Agriculture and life sciences"}]}]},"item_2_description_16":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Alanine aminotransferase from germinating castor bean endosperms was purified about 1,500-fold by ammonium sulfate fractionation and chromatographies with DEAE-Sepharose CL-6B, TEAE-Cellulose, QAE-Sephadex A-50, and TSK-5PW (HPLC). The molecular weight of the enzyme was about 110kDa, with two identical subunits. The purified enzyme was highly specific for alanine as an amino donor and 2-oxoglutarate as an acceptor, and it showed also glyoxylate transamination activities at low rate. The evidence indicates that alanine: 2-oxoglutarate aminotransferase and glyoxylate aminotransferase activities are associated with the same protein. Pyridoxal phosphate as a cofactor was so tightly bound to the enzyme that added pyridoxal phosphate was not required in the assays. The enzyme showed normal Michaelis-Menten kinetics for all substrates. Reactions proceeded by a Ping Pong Bi Bi mechanism. The K_m for L-alanine, 2-oxoglutarate were 3.4mM and 0.26mM, respectively. The optimum pH was 8.5 and pI was about 4.6.","subitem_description_type":"Abstract"}]},"item_2_description_6":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Bulletin of the University of Osaka Prefecture. Ser. B, Agriculture and life sciences. 1995, 47, p.95-104","subitem_description_type":"Other"}]},"item_2_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24729/00009212","subitem_identifier_reg_type":"JaLC"}]},"item_2_publisher_34":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"University of Osaka Prefecture"}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10923100","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0366-3353","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_17":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"FUJII, Michiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"YOSHII, Keiji"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-31"}],"displaytype":"detail","filename":"KJ00004350446.pdf","filesize":[{"value":"579.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00004350446.pdf","url":"https://omu.repo.nii.ac.jp/record/9219/files/KJ00004350446.pdf"},"version_id":"40cb1717-0566-48d8-85ef-9ebf5a2ad2e1"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Purification and Properties of Alanine aminotransferase from Germinating Castor Bean Endosperms","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Purification and Properties of Alanine aminotransferase from Germinating Castor Bean Endosperms"}]},"item_type_id":"2","owner":"1","path":["130","1672"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-08-25"},"publish_date":"2009-08-25","publish_status":"0","recid":"9219","relation_version_is_last":true,"title":["Purification and Properties of Alanine aminotransferase from Germinating Castor Bean Endosperms"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T17:27:09.774776+00:00"}