@article{oai:omu.repo.nii.ac.jp:00009679, author = {MA, Biao and TARUMOTO, Isao and OGAWA, Takeshi and MORIKAWA, Toshinobu}, journal = {大阪府立大学大学院農学生命科学研究科学術報告}, month = {Mar}, note = {application/pdf, To develop an efficient tissue culture method for in vitro bulb formation from leaf explants of Lycoris, regeneration conditions were studied. Higher regeneration rates were obtained using 1 mg/l 1-naphthaleneacetic acid (NAA) with 5 mg/l 6-benzylaminopurine (BAP) in modified MS medium of Huang and Liu (1989). Adventitious buds were induced from the proximal (young) end of explant without intervening callus. Regeneration capacity was greatly affected by ages and parts of leaf, and the basal parts of younger leaves gave higher regeneration rates. In culturing the basal parts of younger leaves on the above optimum medium, 5.0-56.7% regeneration rates with 1.0-11.4 bulblets/explant were obtained in 12 out of 13 tested genotypes. Regenerated bulblets developed into whole plants with bulb after transfer onto hormone-free MS medium., 大阪府立大学大学院農学生命科学研究科学術報告. 2002, 54, p.1-5}, pages = {1--5}, title = {Micropropagation using Leaf Blades in Genus Lycoris}, volume = {54}, year = {2002} }