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長期ホルマリン固定肺組織からのDNA抽出 : PCR法によるPneumocystis carinii DNAの検出(自然科学系)
https://doi.org/10.24729/00010849
https://doi.org/10.24729/000108492bb3bc32-c0ab-46f9-9edb-768269ec4418
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00000202469.pdf (846.4 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2009-08-25 | |||||
| タイトル | ||||||
| タイトル | 長期ホルマリン固定肺組織からのDNA抽出 : PCR法によるPneumocystis carinii DNAの検出(自然科学系) | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Pneumocystis carinii | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | polymerase chain reaction (PCR) | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | formalin-fixed lung tissue | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| ID登録 | ||||||
| ID登録 | 10.24729/00010849 | |||||
| ID登録タイプ | JaLC | |||||
| 著者 |
前河, 裕一
× 前河, 裕一× 中村, 由希子× 竹島, 俊一× 松岡, 喜美子× 中田, 善之× 川端, 邦弘 |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 24549 | |||||
| 姓名 | Maekawa, Yuichi | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 24550 | |||||
| 姓名 | Nakamura, Yukiko | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 24551 | |||||
| 姓名 | Takeshima, Shunichi | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 24552 | |||||
| 姓名 | Matsuoka, Kimiko | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 24553 | |||||
| 姓名 | Nakata, Yoshiyuki | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 24554 | |||||
| 姓名 | Kawabata, Kunihiro | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Pneumonia caused by Pneumocystis carinii (Pc) has been diagnosed by direct detection of Pc in sputum and bronchoalveolar lavage (BAL) collected from patients. In recent years, a method using polymerase chain reaction (PCR) has been more frequently applied to diagnosis of Pc pneumonia. As material for detection of Pc by the PCR method, sputum, BAL, and formalin-fixed tissue are used. Pc genes, however, cannot be detected by PCR in specimens fixed in formalin for a prolonged time. In this study, we improved a method of extracting 'DNA from lung specimens fixed in formalin for a prolonged time. As target genes for amplification, ribosomal RNA internal transcribed spacers, 5S ribosomal RNA, and mitochondrial ribosomal RNA of Pc were selected. As the control for confirming that template DNAs Were appropriate for PCR amplification, human β-globin gene was amplified. Using this method of DNA extraction, in which digestive and fixative removal were promoted, the Pc genes were detected in lung tissue specimens fixed in formalin. Amplification of the control human β-globin gene was also improved, suggesting that Pc detection in fixed lung tissue specimens in this study was due to an increased amount of template DNA by improved DNA extraction. | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 大阪府立看護大学医療技術短期大学部紀要. 2000, 5, p.39-44 | |||||
| 書誌情報 |
大阪府立看護大学医療技術短期大学部紀要 巻 5, p. 39-44, 発行日 2000-03-27 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1341-6421 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN10509373 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| その他のタイトル | ||||||
| その他のタイトル | DNA Extraction from Long Term Formalin-Fixed Lung Tissue : Detection of Pneumocystis carinii DNA by Polymerase Chain Reaction(PCR) Method | |||||
| 出版者 | ||||||
| 出版者 | 大阪府立看護大学医療技術短期大学部 | |||||
